Publications

This poster was produced during the 2004-2007 MfB programme.

The continual development of soft ionization techniques such as electrospray and MALDI have now made the qualitative analysis of oligonucleotides (single stranded DNA fragments) and characterisation of single nucleotide polymorphisms routine analyses. The identification and quantification of large oligonucleotides i.e. >80mer and double stranded DNA is still a challenge to the mass spectrometry community and the former has only really been achieved using FT-ICR/MS. Three areas of research are discussed that address some of the challenges associated with genomic mass spectrometry measurements. The applications presented in this poster represent the tip of the iceberg when it comes to the use of mass spectrometry in genomics. However, we are now beginning to move toward reliable, traceable, quantitative genomic mass spectrometric measurements that may one day allow the direct detection and quantification of DNA molecules without the need for PCR.

This poster aims to portray ways in which we have overcome the difficulties often associated with genomic mass spectrometric measurements by reducing the complexity of the analysis. This is relatively easily achieved by digesting the molecules, using a mixture of enzymatic protocols, into smaller units that are more amenable to MS analysis.

To download the poster click on the related content link below.  For further information contact the NMS helpdesk at LGC.