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Traceable oligonucleotide LC-IDMS quantitation: linking genetic tests and reliable DNA quantitation?
This poster was produced during the 2004-2007 MfB (Measurements for Biotechnology) programme. The work was supported by LGC under project GM2 - Primary methodology for DNA quantitation which fell under the gene measurement theme of projects.
This work compares two different enzymatic digestions for the quantitation of a 20mer oligonucleotide by liquid- chromatography-isotope dilution mass spectrometry (LC-IDMS). The first approach employs one enzymatic digestion using snake venom phosphodiesterase I (SVP) and digests the oligonucleotide down to nucleotides. The second method involves two digestion steps using SVP and shrimp alkaline phosphatase (SAP) and results in the digestion of the oligonucleoside to nucleosides.
The poster can be downloaded by clicking on the related content link below. Further information can be obtained from the NMS helpdesk at LGC.
Last modified on
22 April 2008.