Publications

Production and Fragmentation of Negative Ions from Neutral N-Linked Glycans Ionized by MALDI Mass Spectrometry for Rapid Structural Identification

This poster relates to work carried out under project BM4 - Measurement of key post translational modifications in biopharmaceuticals of the NMS Chemical and Biological Metrology programme.

Fragmentation spectra of negative ions from neutral carbohydrates, as seen by CID following electrospray ionization, contain much more structural information than corresponding spectra of positive ions. However, neutral carbohydrates are reluctant to form negative ions by MALDI except from specific matrices such as nor-harmane ([M-H] - ions) or by forming adducts with chloride or sulphate. None of these ions yield ideal MS or MS/MS spectra. This poster describes an improved method for producing negative ions by doping common matrices with anions such as nitrate and phosphate.

This poster was presented at the 57th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry which was held in Philadelphia in June.

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