Publications

The use of multidimensional image-based analysis to accurately monitor cell growth in 3D bioreactor culture.

The transition from traditional culture methods towards bioreactor based bioprocessing to produce cells in commercially viable quantities for cell therapy applications requires the development of robust methods to ensure the quality of the cells produced. Standard methods for measuring cell quality parameters such as viability provide only limited information making process monitoring and optimisation difficult. Here we describe a 3D image-based approach to develop cell distribution maps which can be used to simultaneously measure the number, confluency and morphology of cells attached to microcarriers in a stirred tank bioreactor.

Further information can be obtained from PLoS One or by contacting the authors via the NMS helpdesk at LGC.