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Evaluation of different analytical strategies for the quantification of sulfur-containing biomolecules by HPLC-ICP-MS: Application to the characterisation of 34S-labelled yeast
This paper evaluates different analytical strategies, such as Compound Independent Calibration (CIC), post-column Isotope Dilution Analysis (IDA) and post-column Isotope Pattern Deconvolution (IPD) for the determination of sulfur-containing biomolecules. CIC graphs were obtained for the sulfur-containing compounds sulfate, cysteine, glutathione and methionine separated by isocratic HPLC using four different ICP-MS instruments. Instruments evaluated included two quadrupole based instruments (XSeries II and HP-4500), with and without a collision cell respectively, one single collector double focusing (Element 2) and one multicollector double focusing (Neptune).
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- Author - Santamaria-Fernandez R
- Reference Number - J. Giner Martínez-Sierra, J. Anal. At. Spectrom., 2010, DOI: 10.1039/b925366a
Last modified on
01 July 2010.