Publications

Isotopic characterisation of in-house purified progesterone for 13C/12C isotope ratios by multicollector ICP-MS

This paper relates to work carried out under Project I3 – Isotope ratio mass spectrometry to establish authenticity and origin of goods of the NMS Chemical and Biological Metrology programme.

In this paper, a multicollector inductively coupled mass spectrometer (MC-ICP-MS) has been used for the first time to characterise a steroid sample for carbon isotope ratio traceable to SI. The progesterone sample was purified in house using semi-preparative high performance liquid chromatography (HPLC) and characterised using a multi-method approach. Carbon ¹³C/¹²C isotope amount ratios have been measured for the progesterone standard and NIST RM materials have been used as QCs to test instrumental performance.

Further information can be obtained from JAAS on the RSC Publishing website or by contacting the authors via the NMS helpdesk at LGC.