Welcome to the NMS chemical and biological metrology website. Please log in
to view restricted content.
Project OA2 – Methods and materials to support clinical laboratory measurements
The development of isotope dilution mass spectrometry methodology for a range of clinical analytes has been funded in previous NMS Programmes, resulting in the availability of methods and Certified Reference Materials (CRMs) for creatinine and electrolytes in serum, testosterone in serum and, under development in the 2006-2009 NMS CBM Programme, digoxin in serum, tacrolimus in blood and trace metals in serum. These materials have been developed in collaboration with organisations heavily involved in clinical laboratory medicine, which have prepared candidate materials and provided confirmatory measurement data.
The need for traceable high-quality CRMs remains strong and is reinforced by the demands of ISO 15189 (Medical Laboratories - Particular Requirements for Competence). For organic analytes, LC-MS is being increasingly used in hospital laboratories, although many still use immunoassay techniques, which are known to have limited traceability and problems with interferences.
Consultation with the Clinical Reference Material User Group (CRMUG) has identified the key clinical analyte/matrix combinations as being of interest:
- Theophylline in serum: Theophylline is a bronchodilatory drug, and accurate monitoring of its concentration in serum is important in assessing patients and facilitating appropriate dosage.
- Cyclosporine in blood: Cyclosporine is an immunosuppressant drug used following organ transplantation, and accurate measurements are necessary to monitor and apply the appropriate patient dosage.
- Buprenorphine in urine or oral fluid: Buprenorphine is a very powerful opiate analgesic, used in the treatment of opiate addiction.
- Vitamin D in serum: Vitamin D deficiency is increasing in the UK, making monitoring and treatment more important. Vitamin D is produced in the body from the pro-hormones D1 and D2, and measurement of the active form is most important.
- Total metals in urine: The monitoring of metals in urine is important in assessing patients for nutrient deficiencies and for diagnosis of causes of toxicity.
Aims and objectives
Last modified on
23 June 2011.